diversity and genetic structure of capoeta trutta (heckel, 1843) populations in the kurdistan province using inter simple sequence repeat markers

inter simple sequence repeat (issr) analysis was performed to evaluate genetic diversity and genetic structure of six capoeta trutta populations from two main watersheds in the kurdistan province. fish samples were caught from the choman, shooei, garmab rivers in the choman watershed and from the gheshlagh, gavarood, sirvan rivers in the sirvan watershed. from six used dinucleotide primers, three ((ag)9c, (ga)9c and (ac)9t)) generated discernible bands. fifty six clear and repeatable bands were generated throughout six populations. the percentages of polymorphism were 80.00, 82.98, 82.61, 85.71, 79.60 and 80.49 within the sirvan, gavarood, gheshlagh, choman, garmab and shooei populations, respectively. the average values of heterozygosites were determined as moderate to high within the studied populations. the intrapopulation genetic diversity indices were significantly higher in the choman population than others.  the analysis of molecular variance revealed 89 percent variation within populations whereas only 11 percent between populations. population comparisons based on fixation index (fst) revealed low to moderate differentiation between six studied populations. however there was no significant differentiation between populations as testified by exact test. the results showed that environmental conditions to maintain genetic diversity of capoeta trutta populations in two watersheds are moderate.